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WEFTA wg meeting 2011
Cesenatico, Italy

The 33nd annual meeting of the WEFTA Working Group on Analytical Methods took place under the chairmanship of H. Karl in Cesenatico, Italy on Thursday May 26th , 2011. The meeting of the “Working Group on Analytical Methods” was attended by 9 representatives from 8 institutions of the following countries: Belgium, Denmark, France (2), Germany (2), Italy (2), Turkey. Apologies for absence were received from Iceland, Spain, Sweden and Croatia.

Adopted Agenda

1. Opening of the meeting (H Karl) and welcome address by host country (S. Testi)

2. Nomination of rapporteur

3. Technical details of the meeting

4. Introduction of new participants

5. Report from the last meeting (Lisbon, 2010)

6. Reports from the last „WEFTA representatives meeting“ in Cesme, Turkey

7. New publications of WG members or institutions of interest for the group (all)

8. Internal reference material – results and conclusions for future work (all) Fish meal (Monika), fatty acid material (Rogerio, Silvia)

9. Tropical and aquaculture species – nutritional value and other issues (all)

10. Parasites progress report + EU call (all)

11. Contaminants progress report + EU call (all)

12. Trace elements (wild – aquaculture) (all)

13. Microbiological issues (all)

14. Actual laboratory question, problems and “good to know” issues

15. Home page – Publication of WEFTA studies and home methods (all)

16. Future business and tasks of the Working Group

17. Other business

18. Date and place of the next meeting

19. Adjourn

1. Opening

The chairman H. Karl opened the meeting and S. Testi and A. Badiani welcomed all the participants in Cesenatico (Facolta di Medicina Veterinaria).

2. Nomination of rapporteur

K. Bekaert was nominated as rapporteur for 2011.

3. Technical details of the meeting

Some details were given on the arrangements for the meeting. S. Testi gave a small presentation of the Faculty of Veterinary Medicine of Bologna. The main task of the Faculty is teaching (Bachelor levels). There are also some research activities going on.

4. Introduction of participants

All representatives gave a presentation on reorganisation, on their field of work and the research projects of interest going on in their institute. Ingrid Undeland and Sanja Vidacek are interested to join the WG, but had other obligations this year.

H. Karl and M. Manthey-Karl - The department of fish quality of the Max Rubner Institute (MRI) will probably be moving to Kiel within the next five years - New projects going on essential elements in milk and fish - Research area changed to metabolomics of fish and nanotechnology - Routine work gets less money, travel budget decreased - Research evolves more towards aquaculture (old things are done on new species) - Contaminant research will decrease in future - Research on parasites and additives - H. Karl will send paper about research on the differences between organically and conventionally aquacultured fish, which will be added to the minutes.

K. Bekaert - New research project on the influence of fishing techniques on fish quality - Cooperation with the German company “Sequid” for optimisation of the Sequid device for freshness determination. - Website of Sequid : www.sequid.com - M. Manthey-Karl will send around an abstract of a conference presentation of Sequid.

G. Duflos - Change of name: Anses instead of Affsa. - Main work: determination of spoilage characteristics (microbiology, freshness, biochemical approach, parasitology)

B. Jørgensen - Reorganisation changed last year, now a division of the National Food Institute - Research focuses more on food technology, yet fish and fish products still play a major role - Image analysis in cooperation with companies for on-line control - Acoustics on shrimps for glazing determination, maybe possible to use the technique to discriminate between single and double frozen fish

S. Testi - Work on quality of fish with the aim of certification on the origin (authenticity of region). - Analysis on FA, minerals, micro/macro-elements, isotopes to identify the region (also difference between wild and aquaculture fish) - Separation between polar and non-polar lipids, Sylvia and B. Poli will send the a flow-sheet of the method to all participants. - P-analysis can be done to understand phospholipid composition.

V. Verrez-Bagnis - Ifremer had a last reorganisation in March: 4 departments. - Véronique is in biggest department: “Biological resources and environment” - In her laboratory “Science and Technique of the Marine Biomass” (head of the lab.: Jean-Pascal Bergé), work is done on transfer of knowledge (Codex alimentarius, revue of scientific papers), by-products, life cycle analysis (C-fingerprint), safety, quality and authenticity. - Finished project: FishPopTrace - New national project on fish parasites with Anses: Fish-Parasites - Travel budget decreased A. Cadun - 1 national project on difference in polyphenol activity of deep-water species in function of time of year, different body parts, …

B. Poli - Beginning of 2010: new department: “Agricultural Biotechnology” with 6 different sections - Section of Bianca: Animal science, - Work is done on aquaculture and quality of fish - G. Parisi works on shellfish (mussels, oysters) and new species (ex. Argyrosomus Regius – Italian denomination Ombrina boccadoro), reared in floating cage or in ponds - Research on the difference between organically and conventionally aquacultured fish - B. Poli did a small project on micro-algae and its use in the nutrition of fish for organic fish production (“greenish seabass” was obtained, no difference in FA composition, but difference in level of diets digestibility). Another project is in running.

5. Report from the last WEFTA representatives meeting

The WEFTA representative’s meeting took place in Cesme, Turkey. The report was distributed at the representative’s meeting. Some topics were proposed by the representatives: micro and macro – algae, nutritional value of aquaculture products, trace elements and Carbon fingerprinting. Nutritional value of aquaculture products Is there a possibility to write a common paper on the data we have on the difference between conventionally and traditionally produced aquaculture products, for example on trout, sea bass in function of rearing conditions, feed, seasons. Some info found in literature could be added. A second paper could be written on the difference between wild and aquaculture fish. Everyone makes an overview of published data and sends them to Monika who will compile the data. A lot of data has not been published yet. It was agreed that at the next meeting, everyone can summarize its “not published data” and present them. Presentations can also be given on the status of aquaculture in the different countries (important species, ecological farming versus conventional), how is it evaluating and what is of interest in the future in this field. Micro-algae An interesting topic can be the nutritional aspect of the use of micro-algae as a feed component in aquaculture. The subject will not be followed by the WEFTA WG, because it is not really of interest at the moment. Integrated aquaculture At the moment, there is no clear link with the analytical WG. A topic could be the influence of integrated aquaculture on fish quality and standardisation of methods. Guiliana Parisi run an experience on integrated aquaculture on fish and shellfish. Next meeting, if she’s willing, she can present this work also implicating standardisation of methods and collaborative trials. Carbon-fingerprinting Only a group of Ifremer is working on this topic (computer modelling of Carbon pathway of by-products of industry).

6. Report of the last meeting of the analytical method working group in Lisbon (2010) The report was accepted without any changes.

7. New publications

Everyone sends its publications/ reports of interest of 2010 to K. Bekaert, who will compile the different papers and send them around . V. Verrez-Bagnis gave an update on the open archive of Ifremer, called archimer (http://archimer.ifremer.fr/), also including reports and PhD thesis’s.

8. Internal Reference Material

An overview was given by H. Karl of the results of the labs participating in the trial. The reference material was used as such (no drying prior to analysis). Water content, fat, protein, salt, ash and TVB were measured. It looks like the material can be used for most of these parameters, with the exception of TVB. Heida will be asked by H. Karl if it is possible for her to make a new badge (10 * 200 g for each lab). The tests will be redone and a Standard Operation Procedure (SOP) will be written. Probably results would be better if the material is dried before using it and so it could serve as an internal reference material.

S. Testi used capelin meal as reference material for fatty acid determination. DHA content is different between Bligh and Dyer and Smedes. Maybe fractionating the lipids first can give better results. The trial will be repeated and the WG is interested to follow-up the results. The material from Heida could be used for FA-analysis.

H. Karl presented a method for Accelerated Solvent Extraction (ASE). He will send the method to K. Bekaert. •

The article on the Smedes method has been accepted by the JAOAC, but there is no further info on the status of publication.

9. Tropical and aquaculture species – nutritional value and other issues (all) National Contact Points were contacted, but we were not successful to have the topic in the new EU-Call. Only the issue on parasites is now in the EU-call. G. Duflos will rewrite the summary draft so it can be send again to the NCP’s and brought forward towards the EU. Issues of interest are quality standardisation, species identification, microbiology, (bio)toxins, deterioration, … MRI continues collecting data on the issue.

10. Parasites progress report + EU call (all)

EU-call Consortium was built outside of the WEFTA WG. Building a new consortium within WEFTA WG will be a waste of time, because a lot of experts are in the first consortium (already 8 à 10 partners like the European Reference Lab, A. Levson, …) Maybe there is an option as subcontractor, but then the results are no longer property of the institute working as subcontractor. Progress Report Ifremer continues its work on a national level: - Work on freshwater fish of the Lehman lake (e.g. in perch ) - Work on the 15 most important fresh species on the French market (salmon, sole, sea bass, mackerel, herring, hake, … ) o Anisakis o Cryptosporidium o Copepods - Germany did research on the most important species on the German market (Alaska Pollack, herring, salmon, canned tuna, pangasius) - Ifremer received a question from industry on Kudoa in canned tuna. Kudoa is a parasite which softens the flesh of the fish after death. It is also found in mackerel, salmon and fish. - Germany updated on work on grey gurnard in collaboration with A. Levsen. The species is very abundant, but is also heavily infected by anisakid nematodes (typical for predators). MRI also trains control instances to detect nematodes and organize ring tests. - The progress will be followed during the next WG meeting.

11. Contaminants progress report + EU call (all)

Update from Germany: no real decrease in dioxin or dioxin-like PCB’s any more. There was a big decrease from 1980, now it reached the background level. 12. Trace elements (wild – aquaculture) (all) Contributions from France and Denmark were sent to J. Luten. B. Poli will contact project partners to see if she can send information (not her own data) to J. Luten. The aim is to write a paper on it. S. Testi will contact J. Luten to ask if he is still interested in new data. 13. Microbiological issues (all) There were no microbiological issues. 14. Actual laboratory question, problems and “good to know” issues - Update on Accelerated Solvent Extraction by H. Karl. - G. Duflos asks if there is interest for validation – standardisation of the histamine determination by HPLC-method. He obtained a project from the European Committee for Standardisation (CEN) from 2009. - 2 parts in the project: o Constitution of a working group who should organize the sampling design and the ring test (start in September). Different representatives of laboratories should be member of the working group. o Realisation of the validation and ring test. - G. Duflos will send around an e-mail with a summary of the project so that it can be sent to the responsibles of the analysis in the different labs.

15. Home page – Publication of WEFTA studies and home methods (all)

K. Bekaert made a template and will adapt it following the remarks from the WG. Everyone fills in the template for the validated home methods who could be published on the WEFTA home page. Everyone sends it home methods to H. Karl who will pass them to J. Luten for publication on the Wefta home page. M. Manthey-Karl will ask Jörg for old publications about ring trials realised by the Wefta WG. She will write an abstract of the principle of the methods.

16. Future business and tasks of the Working Group

See detailed information under the different topics above.

17. Date and place of the next meeting

Thursday 7th of June, Izmir, Turkey. Details for lodging and travelling will be delivered later.

18. Other business

None

19. Adjourn

The chairman expressed his thanks to the group and considered this meeting a very fruitful and successful one. Then he closed the meeting.